Biochem/physiol Actions

Cell permeable: no

Reversible: no

Primary Targetinactivates RNase

Product does not compete with ATP.

General description

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T₁ and S1 nuclease from Aspergillus.

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T₁ and S1 nuclease from Aspergillus.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.Lee, F.S., et al. 1989. Biochemistry 28, 225.

Packaging

2500 u in Plastic ampoule

Physical form

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Reconstitution

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Unit Definition

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

Warning

Toxicity: Standard Handling (A)